一、个人简介

方龙发，男，籍贯安徽安庆，博士，青年研究员，硕士生导师

研究方向：紫花苜蓿耐逆修饰组学研究、新型基因编辑工具的开发，紫花苜蓿耐逆育种。

邮箱：fanglf@lzu.edu.cn; fanglongfa@126.com

通讯地址：甘肃省兰州市城关区嘉峪关西路768号兰州大学草地农业科技学院，邮编：730020

二、教育背景与工作经历

2020.07-至今，兰州大学，草地农业科技学院，青年研究员，草类植物育种与种子研究所

2014.09-2020.01，中国科学院大学，生物信息学，博士，导师：汪迎春研究员

2011.09-2014.06，中国农科院植物保护研究所和安徽农业大学联合培养，微生物学，硕士，导师：张杰研究员，黃勃教授

2007.09-2011.06，安徽农业大学，生物技术，学士

三、科研项目

[1] 国家自然科学基金青年科学基金项目(32201463)，“MsAPK1蛋白磷酸化修饰调控紫花苜蓿耐旱性的作用机理”，30万元，2023.01-2025.12，主持

[2] 国家重点研发计划（2023YFF1001504），“农业生物重要性状形成与环境适应性基础研究”，子课题负责人，110万元，2023.11-2027.11, 子课题主持

[3] 甘肃省重大科技专项，“高寒草地草种创新、生态修复与高效草地农业系统研究”，14万元，2022-2025，子课题主持

[4] “双一流”引导专项-队伍建设经费-科研启动费，100万元，2020.09-2025.08，主持

[5] 保密项目, 250万元，2022-2027，子课题主持

四、主要科研成果

一作或通讯发表文章：

[1] Han, Y., Ge, H., Xu, C., Zeng, G., Li, Z., Huang, X., Zhang, Y., Liu, Z., Wang, Y., and Fang, L. (2024). Glycosyltransferase Slr1064 regulates carbon metabolism by modulating the levels of UDP‐GlcNAc in Synechocystis sp. PCC 6803. New Phytologist. （生物学1区，主流期刊，9.4）

[2] Fang, L., Li, M., Zhang, J., Jia, C., Qiang, Y., He, X., Liu, T., Zhou, Q., Luo, D., and Han, Y. (2024a). Chromosome‐level genome assembly of Pedicularis kansuensis illuminates genome evolution of facultative parasitic plant. Molecular Ecology Resources, e13966.（生物学1区，7.7）

[3] Fang, L., Liu, T., Li, M., Dong, X., Han, Y., Xu, C., Li, S., Zhang, J., He, X., and Zhou, Q. (2024b). MODMS: a multi-omics database for facilitating biological studies on alfalfa (Medicago sativa L.). Horticulture Research 11, uhad245.（农林1区，8.7）

[4] Qiang, Y., He, X., Li, Z., Li, S., Zhang, J., Liu, T., Tursunniyaz, M., Wang, X., Liu, Z., and Fang, L. (2023). Genome-wide identification and expression analysis of the response regulator gene family in alfalfa (Medicago sativa L.) reveals their multifarious roles in stress response. Frontiers in Plant Science 14, 1149880. （生物学2区，6.7）

[5] Fang, L., Ge, H., Huang, X., Liu, Y., Lu, M., Wang, J., Chen, W., Xu, W., and Wang, Y. (2017). Trophic mode-dependent proteomic analysis reveals functional significance of light-independent chlorophyll synthesis in Synechocystis sp. PCC 6803. Molecular Plant 10, 73-85. （生物学1区, 主流期刊 27.5）

[6] Fang, L., Wang, B., Zhou, Z., Yang, S., Shu, C., Song, F., Bravo, A., Soberón, M., and Zhang, J. (2016). Oligomerization of Cry9Aa in solution without receptor binding, is not related with insecticidal activity. Electronic Journal of Biotechnology 19, 54-57.（生物学4区，2.4）

共同一作或通讯发表文章：

[7] Mingyu Li, Jun Pu, Chenglin Jia, Dong Luo, Qiang Zhou, Xiangling Fang, Bin Nie, Wenxian Liu, Zhibiao Nan, Iain Robert Searle, Longfa Fang*, Zhipeng Liu*. The genome of Vicia sativa ssp. amphicarpa provides insights into the role of terpenoids in antimicrobial resistance within subterranean fruits. The Plant Journal. （共同通讯，生物学1区，6.0）

[8] Yang, T., Li, Z*., Shu, J., Fang, L*., He, X., Li, Q., Jiang, G., Chen, S., and Yang, B. (2023). Rapid Detection of Potential Natural Food Preservatives and Identification of Artemisia Species via High-Sensitivity Photoionization Mass Spectrometry. Journal of Agricultural and Food Chemistry 71, 6197-6209. （共同通讯，农林1区，6.1）

[9] Chen, W., Fang, L.*, Huang, X., Ge, H., Wang, J., Wang, X., Zhang, Y., Sui, N., Xu, W., and Wang, Y. (2018). Systematic identification of light-regulated cold-responsive proteome in a model cyanobacterium. Journal of Proteomics 179, 100-109.（共同一作，蛋白质组学主流期刊，2.8）

[10] Ge, H*., Fang, L*., Huang, X*., Wang, J., Chen, W., Zhang, Y., Wang, X., Sui, N., Xu, W., and He, Q. and Wang, Y. (2018). Activation of the oxidative pentose phosphate pathway is critical for photomixotrophic growth of a hik33-deletion mutant of Synechocystis sp. PCC 6803. Proteomics 18, e1800046. （共同一作，蛋白质组学主流期刊，3.4）

参与发表的文章

[11] Dong, X., Chen, J., Zhou, Q., Luo, D., Fang, L., Liu, W., and Liu, Z. (2024). Pod-shattering characteristic differences between shattering-resistant and shattering-susceptible common vetch accessions are associated with lignin biosynthesis. Journal of Integrative Agriculture.

[12] Yang, T., Li, Z., Chen, S., Lan, T., Lu, Z., Fang, L., Zhao, H., Li, Q., Luo, Y., and Yang, B. (2024). Ultra-sensitive analysis of exhaled biomarkers in ozone-exposed mice via PAI-TOFMS assisted with machine learning algorithms. Journal of Hazardous Materials 470, 134151.

[13] Zhou, Q., Cui, Y., Dong, S., Luo, D., Fang, L., Shi, Z., Liu, W., Wang, Z., Nan, Z., and Liu, Z. (2023). Integrative physiological, transcriptome, and metabolome analyses reveal the associated genes and metabolites involved in cold stress response in common vetch (Vicia sativa L.). Food and Energy Security 12, e484.

[14] Luo, D., Zhang, X., Liu, J., Wu, Y., Zhou, Q., Fang, L., and Liu, Z. (2023). DROUGHT-INDUCED UNKNOWN PROTEIN 1 positively modulates drought tolerance in cultivated alfalfa (Medicago sativa L.). The Crop Journal 11, 57-70.

[15] Luo, D., Liu, J., Wu, Y., Zhang, X., Zhou, Q., Fang, L., and Liu, Z. (2022). NUCLEAR TRANSPORT FACTOR 2‐LIKE improves drought tolerance by modulating leaf water loss in alfalfa (Medicago sativa L.). The Plant Journal 112, 429-450.

[16] Zhou, Q., Mao, P., Luo, D., Chai, X., Deng, H., Fang, Q., Fang, L., Nan, Z., Wen, J., and Liu, Z. (2022b). Comparative transcriptome analyses reveal that the MsNST1 gene affects lignin synthesis in alfalfa (Medicago sativa L.). The Crop Journal 10, 1059-1072.

[17] Zhou, Q., Cui, Y., Dong, R., Luo, D., Fang, L., Nan, Z., and Liu, Z. (2022a). Integrative analyses of transcriptomes and metabolomes reveal associated genes and metabolites with flowering regulation in common Vetch (Vicia sativa L.). International Journal of Molecular Sciences 23, 6818.

[18] Wu, Y., Luo, D., Fang, L., Zhou, Q., Liu, W., and Liu, Z. (2022). Bidirectional lncRNA transfer between Cuscuta parasites and their host plant. International Journal of Molecular Sciences 23, 561.

[19] Wang, J., Huang, X., Ge, H., Wang, Y., Chen, W., Zheng, L., Huang, C., Yang, H., Li, L., Sui, N, Fang, L., Wang，Y. (2022). The quantitative proteome atlas of a model cyanobacterium. Journal of Genetics and Genomics 49, 96-108.

[20] Wang, Z., Fang, L., Zhou, Z., Pacheco, S., Gomez, I., Song, F., Soberon, M., Zhang, J., and Bravo, A. (2018). Specific binding between Bacillus thuringiensis Cry9Aa and Vip3Aa toxins synergizes their toxicity against Asiatic rice borer (Chilo suppressalis). Journal of Biological Chemistry 293, 11447-11458.

[21] Ge, H., Fang, L., Huang, X., Wang, J., Chen, W., Zhang, Y., Wang, X., Sui, N., Xu, W., and He, Q. (2018). Activation of the Oxidative Pentose Phosphate Pathway is Critical for Photomixotrophic Growth of a hik33‐Deletion Mutant of Synechocystis sp. PCC 6803. Proteomics 18, 1800046.

[22] Gao, L., Wang, J., Ge, H., Fang, L., Zhang, Y., Huang, X., and Wang, Y. (2015). Toward the complete proteome of Synechocystis sp. PCC 6803. Photosynthesis research 126, 203-219.

[23] 方龙发, 王小奇, 束长龙, 等. Cry9Aa3 蛋白对小菜蛾和亚洲玉米螟的最小活性区研究[J]. 植物保护, 2015, 41(2): 80-85.

五、获奖情况

2016年 中国科学院遗传与发育生物学研究所益海嘉里奖-优秀博士生奖；

2016年 中国科学院研究生院三好学生。

六、学生培养

当代科学研究和技术发明变得越来越复杂,进行多学科交叉会有更为重大的发现！热忱欢迎生物学、计算机（生物信息学）、草学、农学等相关专业背景的本科生报考硕士研究生，同时欢迎本科生参加毕业设计。